A single nucleotide polymorphism in XRCC4 gene is associated with reduced colorectal cancer susceptibility in female

ObjectiveTo investigate the association of XRCC4 polymorphic variants at G-1394T (rs6869366) with colorectal cancer susceptibility.MethodsIn this hospital-based case-control study, the association of XRCC4 polymorphism with colorectal cancer risk in Chinese population was investigated. In total, 171 patients with colorectal cancer and 171 healthy individuals matched for age and gender were selected. The genomic DNAs of the patients and controls were extracted from peripheral blood and the 300 bp target DNA was amplified with Polymerase Chain Reaction. The products were then digested with restriction endonuclease HincII, followed by agarose electrophoresis to identify the genotype.ResultsWe found a significant difference in the frequency of the XRCC4 G-1394T genotype between the colorectal cancer and control groups in female (1/127 vs 8/122, P<0.05). Those with G/T at XRCC4 G-1394T showed a decreased risk of colorectal cancer susceptibility compared with those with T/T (OR 0.113, 95%CI 0.014–0.932). However, in overall population or in male, there was no significant difference of the distribution between the colorectal cancer and control groups.ConclusionOur findings suggested that the G allele of XRCC4 G-1394T were associated with decreased risk of colorectal cancer susceptibility in female.