Differential expression of βig-h3 in human hepatoma cell lines1

ObjectiveTo observe the differential expression of TGF-β-induced gene human clone 3 (βig-h3) in human hepatoma cell lines.MethodsHuman hepatoma cells HHCC, 7721, T7721 constructed by stably transfecting HAb18G/CD147 cDNA into 7721 and normal human liver cell QZG were cultured as previously. RT-PCR and western blot were used to investigate the differential expression of βig-h3 in human hepatoma cell lines and normal liver cell.ResultsThe results of RT-PCR suggested that the expression of βig-h3 mRNA in human hepatoma cells was higher than that in normal human liver cell QZG (P < 0. 01), and its expression level in human hepatoma cells in turn was HHCC>T7721>7721. Moreover, the similar results of βig-h3 protein expression were testified by western blot.ConclusionThis study demonstrates that the expression of βig-h3 in hepatoma cell lines is higher than that in normal liver cell QZG, which provides a sound basis for exploring the function of βig-h3 in processes of adhesion and metastasis of human hepatoma cells.