Diagnostic significance of salivary testosterone measurement revisited: using liquid chromatography/mass spectrometry and enzyme-linked immunosorbent assay

Salivary testosterone measured by LC-MS and ELISA is a non-invasive, reliable substitute for serum calculated free or bioavailable testosterone. Considering its cost advantage and technical convenience, ELISA for salivary testosterone is now recommended for the purpose of screening the androgen bioavailability level especially in a large population-based study.