Expression of Beclinl in non small cell lung cancer and its clinical significance

ObjectiveTo investigate the expression of autophagy related gene; Beclinl in human non-small cell lung cancer(NSCLC) tissues.MethodsProtein expression of Beclinl was determined by immunofluorescence staining and Western Blot, mRNA expression was analyzed by RT-PCR.ResultsImmunofluorescence staining revealed that the level of Beclinl expression in lung cancer was significantly lower than that in adjacent noncancerous tissues and normal tissues(expression rate 8.3%, P=0.000). The Beclinl mRNA expression in lung cancer, adjacent noncancerous tissues and normal tissues was 1.372 (+ 0.475) 1.721 (+ 0.521) and 1.553(+ 0.554) when F = 15.0, P < 0.01. Beclinl protein expression in lung cancer, adjacent noncancerous tissues and normal tissues was 3.453(+ 0.852) 5.423(+ 1.351) and 6.878(+0.997) F= 11.2, P < 0.01.ConclusionThe protein and mRNA expression of Beclinl in lung cancer was much lower than those in and around cancer tissues and normal tissues, those differences having statistical significance. However in adjacent noncancerous tissues and normal tissues, the expression showed no difference.