Effects of Oxymatrine on the NF-kappa B expression of HaCaT cells

ObjectiveTo study the effect of oxymatrine on the expression of nuclear factor-kappa B(NF-κB) of Human benign epidermal keratinocytes line(HaCaT cells).MethodsHaCaT cells were cultured with different concentration of Oxymatrine(10 μg/ml, 50 μg/ml and 100 μg/ml) for 24 h, then 10−5 mol/L Substance P was added to the cells. After 30 min, NF-κB expression in the cells was observed by immunocytochemistry, NF-κB P65 protein expression was evaluated by Western blot, and the mRNA expression of NF-κB P65 was evaluated by reverse transcription polymerase chain reaction(RT-PCR). The 10−5 mol/L Substance P and culture medium were added to the Substance P group and control group, respectively.ResultsIn control group, expression rate of positive cells, the expressions of protein and mRNA of NF-κB were all low. In Substance P group, when 10−5 mol/L Substance P was added, the expres-sions were all increased(P < 0.05). But in Oxymatrine groups, the expression rate of positive cells, the expressions of protein and mRNA were all descended in a concentration-dependent manner(P < 0.05 or P < 0.01).ConclusionOxymatrine can down-regulate the expression of NF-κB of the HaCaT cells and may play an important role in regulating anti-inflammation and immunity.