The effect of spinal cord injury on the expression of TGF-β and TNF-α in rat articular cartilage1

ObjectiveTo observe the expression of TGF-β and TNF-α in the spinal cord injured rat model and discuss the significance of the articular cartilage metabolism.Methods36 SD female rats were randomly divided into 2 groups: Rats models of spinal cord injury were implemented by Allen method. T10 laminectomy was performed in the control group. Both groups of rats were killed respectively in 1w, 3w and 6w. Hematoxylin-eosin stain was given to each slice in the model group and control group. Immunohistochemical stain was applied by using ABC method in the expression of TGF-β and TNF-α Those expressed level were performed in image analysis and statistics process.ResultsTGF-β and TNF-α were mainly distributed on the surface layer of the articular cartilage, with a weak expression in control group. The expression of TNF-α in the model group was more significant than that in the control group in the 1w, and still remained an evident difference with that in control group until the 6w (P<0.05). TGF-β expression of the model group had no remarkable difference with the control group in the 1w (P>0.05) and prominently became stronger at 6w (P<0.05).ConclusionThe expression of TNF-α occurred early in the development of spinal cord injury, and the expression of TGF-β became stronger with the revival of spinal neural function. Both expressions are strengthened in articular cartilage in the 3rd week.