Over-expression of hypoxia-inducible factor 1 alpha increases angiogenesis of LNCaP cells

ObjectiveTo evaluate the effect of HIF-1 ± over-expression on angiogenesis in human prostate cancer cells.MethodsLNCaP cells(a human prostate cancer cell line) were transfected with the recombinant plasmid pcDNA3.1(-)-HIF-1 α with Lipofectamine 2000 system. The positive clones were selected by G418 being further confirmed by Western blot and immunofluorescence. The expression levels of VEGF, iNOS and Ang- II were determined.ResultsThe expression of HIF-1 α in the LNCaP/HIF1 α cells was significantly increased in transfected cells, which induced the up-regulation of VEGF, iNOS, whereas Ang- II expression remained un-changed.ConclusionOverexpression of HIF-1 α can induce angiogenesis proteins and may improve the angiogenesis potency of prostate cancer.