کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3484795 | 1596856 | 2006 | 10 صفحه PDF | دانلود رایگان |

Two common polymorphisms of the methylenetetrahydrofolate reductase (MTHFR) gene, the thermolabile C677T and A1298C polymorphism may contribute to hyperhomocysteinemia, a known risk factor for vascular diseases. Twenty with coronary artery disease (CAD) and 20 patients with cerebro-vascular stroke (CVS) were compared with 20 controls. Using PCR and restriction fragment length polymorphism (RFLP) analysis, we studied C677T and A 1298C MTHFR genotypes and their combined effect on homocysteine, measured by chemiluminescent enzymatic immunometric assay. Homocysteine values were significantly higher in CAD (16.12 ± 5.09 μmol/L) and in CVS (16.79 ± 5.93 μmol/L) compared with controls (10.43 ± 2.57 μmol/L, P < 0.01). In C677T genotype, homocysteine was significantly higher in TT (18.26 ± 2.75 μmol/L) and in CT (17.60 ± 7.22 μmol/L) than in CC genotype (12.94 ± 4.16 μmol/L, P < 0.01). However, in A1298C genotype, no significant difference was found between the mean homocysteine level in AA genotype (14.14 ± 4.32 μmol/L), AC genotype (14.25 ± 5.50 μmol/L) and CC genotype patients (16.28 ± 8.76 μmol/L, P > 0.05). A significant positive correlation between plasma homocysteine and cholesterol (r = 0.37, P < 0.01) and LDL-C levels was found (r = 0.321, P < 0.05). The percentage of patients with high homocysteine level (≥15 μmol/L) were significantly higher in CAD (50%) and CVS (55%) than controls (5%, P < 0.01, odd ratio = 21). In contrast to the A1298C polymorphism, the MTHFR TT and CT genotypes were associated with hyperhomocysteinemia. The knowledge of the MTHFR mutation (C677T) status might represent a way to identify subjects at high risk for hyperhomocysteinemia.
Journal: Journal of Taibah University Medical Sciences - Volume 1, Issue 1, 2006, Pages 20–29