کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3485784 1596934 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of chemokine receptor-4 in bone marrow mesenchymal stem cells on experimental rat abdominal aortic aneurysms and the migration of bone marrow mesenchymal stem cells with stromal-derived factor-1
ترجمه فارسی عنوان
بیان ژن گیرنده شیمیایی 4 در سلول های بنیادی مزانشیمی مغز استخوان بر روی آنوریسم های آئورت شکمی تجربی و مهاجرت سلول های بنیادی مزانشیمی مغز استخوان با فاکتور 1 حاصل از استروما
کلمات کلیدی
آنوریسم آئورت شکمی سلول های بنیادی مزانشیمی مغز استخوان گیرنده 4 شیمیایی، عامل مشتق استروما 1
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
چکیده انگلیسی

This study investigated the expression and role of chemokine receptor-4 (CXCR4) in bone marrow mesenchymal stem cells (BMSCs) from experimental rats with abdominal aortic aneurysms (AAA) for migration of BMSCs. Sprague–Dawley rats were divided into an experimental group and control group (n = 18 each). AAA was induced with 0.75 M solution infiltrate for 30 minutes, after which the abdomen was rinsed and closed. Saline was used in place of CaCl2 in the control group. CD34 and CD29 were detected by flow cytometry, the gene and protein expression of CXCR4 were detected by real-time polymerase chain reaction and western blot, respectively. The migration of BMSCs with stromal-derived factor-1 was detected by Transwell chamber. CD34 expression was negative and CD29 expression was positive. The gene and protein expression of CXCR4 were significantly higher in experimental group than them in control group (p < 0.05), the migration ability of BMSCs from the experimental group was significantly higher than that from the control group (p < 0.05). Stromal-derived factor -1/CXCR4 can enhance the migration of BMSCs in vitro in a rat AAA model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Kaohsiung Journal of Medical Sciences - Volume 30, Issue 5, May 2014, Pages 224–228
نویسندگان
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