کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3841805 1248003 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Estrogen and G protein-coupled estrogen receptor agonist G-1 cause relaxation of human gallbladder
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Estrogen and G protein-coupled estrogen receptor agonist G-1 cause relaxation of human gallbladder
چکیده انگلیسی

ObjectiveEstrogen interacts with a membrane receptor, G protein-coupled estrogen receptor (GPER). It was reported that 17β-estradiol was able to inhibit contraction of the human colon and cause relaxation of the guinea pig gallbladder, however, the involvement of GPER was not clarified. The aim of the present study was to investigate the effect of estrogen on human gallbladder motility and the possible role of GPER.Materials and MethodsRelaxation of human gallbladder strips were measured using isometric transducers. Expression of GPER was evaluated by reverse transcription polymerase chain reaction (PCR), real-time PCR, and immunohistochemistry.ResultsIn human gallbladder strips, 17β-estradiol and G-1 elicited marked and rapid relaxation, whereas tamoxifen produced mild concentration-dependent relaxation. The relative efficacies to cause relaxation were as follows: 17β-estradiol = G-1 > tamoxifen. The relaxant response of 17β-estradiol was not attenuated by tetrodotoxin or conotoxin GVIA. This implies that nerve stimulation was not involved in the 17β-estradiol-induced gallbladder relaxation. Analysis by reverse transcription PCR and real-time PCR showed that GPER was expressed in the human gallbladder. Further analysis by immunohistochemistry revealed that GPER was expressed in the gallbladder muscle. This suggests that 17β-estradiol relaxes the human gallbladder via GPER.ConclusionThese results demonstrate for the first time that 17β-estradiol and GPER agonist G-1 cause relaxation of the human gallbladder, probably through GPER. Estrogen might play an important role in the control of human gallbladder motility.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tzu Chi Medical Journal - Volume 28, Issue 2, June 2016, Pages 54–58
نویسندگان
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