کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3884632 | 1249483 | 2011 | 10 صفحه PDF | دانلود رایگان |
Excessive intraperitoneal absorption of glucose during peritoneal dialysis has both local cytotoxic and systemic metabolic effects. Here we evaluate peritoneal dialysis solutions containing l-carnitine, an osmotically active compound that induces fluid flow across the peritoneum. In rats, l-carnitine in the peritoneal cavity had a dose-dependent osmotic effect similar to glucose. Analogous ultrafiltration and small solute transport characteristics were found for dialysates containing 3.86% glucose, equimolar l-carnitine, or combinations of both osmotic agents in mice. About half of the ultrafiltration generated by l-carnitine reflected facilitated water transport by aquaporin-1 (AQP1) water channels of endothelial cells. Nocturnal exchanges with 1.5% glucose and 0.25% l-carnitine in four patients receiving continuous ambulatory peritoneal dialysis were well tolerated and associated with higher net ultrafiltration than that achieved with 2.5% glucose solutions, despite the lower osmolarity of the carnitine-containing solution. Addition of l-carnitine to endothelial cells in culture increased the expression of AQP1, significantly improved viability, and prevented glucose-induced apoptosis. In a standard toxicity test, the addition of l-carnitine to peritoneal dialysis solution improved the viability of L929 fibroblasts. Thus, our studies support the use of l-carnitine as an alternative osmotic agent in peritoneal dialysis.
Journal: Kidney International - Volume 80, Issue 6, 2 September 2011, Pages 645–654