کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3884811 | 1249490 | 2007 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Ephrin-B1 localizes at the slit diaphragm of the glomerular podocyte Ephrin-B1 localizes at the slit diaphragm of the glomerular podocyte](/preview/png/3884811.png)
Ephs and ephrins are a family of membrane-bound proteins that function as receptor–ligand pairs. Members of the Eph–ephrin-B family have recently been reported to regulate the paracellular permeability of epithelial cells. In this study, we analyzed the expression and the function of ephrin-B1 in glomeruli. Using immunofluorescence (IF), we found that ephrin-B1 was expressed along the glomerular capillary loop. Immunoelectron microscopy revealed that ephrin-B1 expression was restricted at the slit diaphragm. Dual labeled IF showed ephrin-B1 colocalized with the slit diaphragm proteins nephrin and CD2-associated protein. Ephrin-B1 colocalized with nephrin at the late capillary loop stage of kidney development. Additionally, injection of rats with a nephritogenic anti-nephrin antibody (ANA) reduced ephrin-B1 expression. When podocytes were cultured in vitro, they extruded processes that co-stained for ephrin-B1 and for CD2-associated protein. When these podocytes were treated in culture with small interfering RNA for ephrin-B1, CD2-associated protein was reduced in the processes, with a remaining faint perinuclear staining. We suggest that ephrin-B1 has a role in maintaining barrier function at the slit diaphragm.
Journal: Kidney International - Volume 72, Issue 8, 2 October 2007, Pages 954–964