کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3887023 1249571 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microinjection and electroporation of embryonic kidney explants: An improved method
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی بیماری‌های کلیوی
پیش نمایش صفحه اول مقاله
Microinjection and electroporation of embryonic kidney explants: An improved method
چکیده انگلیسی

Embryonic kidney explants are routinely used to study the molecular regulation of kidney development. One of the major technical challenges has been the need to express transgenes at high levels for prolonged periods of time. Existing protocols derived from work with the chick have used microinjection and electroporation with low voltage and long pulse time. In this study, we show that a high voltage with a short pulse time is preferable for mouse kidney explants. Using these conditions, gene expression is enhanced 10-fold over a 96-h period in culture with minimal toxicity. Furthermore, by modifying the site of microinjection, the ureteric bud or the metanephric mesenchyme can be targeted. We suggest that our described conditions will make microinjection and electroporation a more effective method to study gene function in the developing mouse kidney.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Kidney International - Volume 72, Issue 1, 1 July 2007, Pages 121–125
نویسندگان
, , , ,