کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3913675 1251445 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Low-dose mifepristone increases uterine expression of aquaporin 1/aquaporin 2 at the time of implantation
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Low-dose mifepristone increases uterine expression of aquaporin 1/aquaporin 2 at the time of implantation
چکیده انگلیسی

BackgroundThe aim of this study was to investigate the mechanism by which low-dose mifepristone serves as an antiimplantation contraceptive drug. A human endometrial explant system was used to study the effects of low-dose mifepristone (65 nmol/L and 200 nmol/L) on expression of the water channel family aquaporins, aquaporin-1 and aquaporin-2 (AQP1/AQP2), at the time of implantation.Study DesignEndometrial samples from 17 normally cycling patients at the “window of implantation” were treated with different concentrations of mifepristone. The protein and mRNA expression of AQP1/AQP2 in the endometrium was examined using immunohistochemistry (IHC) and reverse transcriptase–polymerase chain reaction (RT-PCR), respectively.ResultsThe IHC and RT-PCR analyses demonstrated that expression of AQP1/AQP2 was increased by mifepristone in a dose-dependent manner, with the highest AQP1/AQP2 expression levels detected in subjects treated with 200-nmol/L mifepristone.ConclusionLow-dose mifepristone may negatively regulate implantation by increasing AQP1/AQP2 protein and mRNA expression. The findings from this study provide further evidence to support the potential contraceptive activity of low-dose mifepristone.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Contraception - Volume 87, Issue 6, June 2013, Pages 844–849
نویسندگان
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