کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3919454 1599784 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The impact of blastomere survival rates on developmental competence of cryo-thawed Day 2 embryos
ترجمه فارسی عنوان
تأثیر میزان بقای بلاستومر بر مهارت های رشد جنین های روز دوم خرد شده
کلمات کلیدی
تنها انتقال جنین، بقای جنین، از دست دادن سلول
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
چکیده انگلیسی

ObjectivesThe aim of the present study is to investigate the effect of embryonic blastomere loss, following cryopreservation and thaw of Day 2 embryos in an assisted reproductive technology (ART) setting, on pregnancy outcome and fetal development.Study designThis is a retrospective analysis performed on 3553 slow frozen-thawed Day 2 embryos, of all IVF/ICSI thawing cycles carried out during the 11 year study period. Of these thawed embryos, 628 underwent SET on Day 3 of embryo development. We measured the influence of several laboratory parameters on blastomere loss after thaw including: cell stage at cryopreservation, blastomere loss post-thaw, ability to resume mitosis and the rate of overnight cleavage.ResultsThere is an association between cell number on day of freeze and embryonic survival post-thaw; 3 cell (77.4%), 4 cell (92.1%), 5 cell (81.4%) and 6 cell (86.5%) embryos (p < 0.05). We found a significant association between the rate of overnight cleavage and positive hCG and implantation rate (p-value <0.05), while there is no association with live birth rate (p-value 0.242). Embryos with 100% blastomere survival have significantly higher cleavage rates, positive hCG, implantation and live birth rates than embryos which experienced blastomere loss (p < 0.05). However, blastomere survival has no impact on miscarriage rate or the observed newborn birth weight (3.85 ± 0.77 kg).ConclusionsIn the present study we demonstrate, for the first time, that although it is optimal to select an embryo with 100% blastomere survival, transfer of an embryo with ≥50% blastomeres intact post-thaw does not influence the development of the baby, as indicated by weight at birth.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology - Volume 197, February 2016, Pages 98–102
نویسندگان
, , , ,