Operator experience reduces the risk of second trimester amniocentesis-related adverse outcomes

ObjectiveTo investigate the impact of operator experience on amniocentesis-related adverse outcomes.Study designRetrospective study of mid-trimester amniocenteses performed by the same operator on singleton pregnancies in a single private institution during 1994–2007. Outcomes were hemorrhagic or dark amniotic fluid aspiration, insufficient volume aspiration, repeated puncture and fetal loss. Rates were estimated annually, as well as for every 10% of procedures up to the total number. The association of each outcome with epidemiological aspects was also examined.ResultsIn total, 5913 amniocenteses were performed. The overall rate of adverse outcomes was 5.4%. The total adverse outcome rate reduced from 10.2% in the first 10% of cases to 3.0% in the last 10% (P = .001). The rate of hemorrhagic fluid gradually decreased from 4.4% to 1.5% (P = .05) over the same intervals. The fetal loss rate was also reduced from 0.5% during the first half to 0.3% in the second half of the study period (P = NS). Logistic regression analysis indicated no significant correlations between adverse outcomes with any of epidemiological parameters of women undergoing amniocentesis.ConclusionOperator experience has a beneficial impact on preventing procedure-related adverse outcomes.