کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3920338 | 1599825 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Secreted cyclophilin A induction during embryo implantation in a model of human trophoblast–endometrial epithelium interaction Secreted cyclophilin A induction during embryo implantation in a model of human trophoblast–endometrial epithelium interaction](/preview/png/3920338.png)
ObjectiveEmbryo implantation in the human is a complex process that is crucial for a successful pregnancy. Implantation involves complex interactions between the embryo and the maternal endometrium. In order to understand the critical events involved in human embryo implantation, we established a human trophoblast–endometrial interaction model to study the putative alterations of gene expression during implantation.Study designComparative proteomic analysis was applied to the co-culture and separated culture systems of the trophoblast cell line BeWo and human endometrial epithelial cell (EEC) line RL95-2. Comparing the secreted molecules resulted in an interesting finding that secreted cyclophilin A (CypA) was increased in the co-culture system. To further verify our observation, human recombinant CypA was applied to endometrial cells to understand the downstream gene regulation.ResultsCyclophilin A (CypA) was identified by MALDI-TOF Mass with higher expression levels in the co-cultured cells when compared to the endometrial cells alone. Western blot analysis further confirmed the increased expression of CypA in co-culture conditions. Immunoblotting analysis showed that both p38 MAPK and extracellular signal-regulated kinase (ERK) were activated in EEC.ConclusionOur results suggest that the secreted CypA plays a specific role in the signaling pathway of embryo implantation. The identification of CypA opens a new avenue for early embryo implantation research.
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology - Volume 164, Issue 1, September 2012, Pages 55–59