کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3920341 | 1599825 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Anal human papillomavirus DNA in women at a colposcopy clinic Anal human papillomavirus DNA in women at a colposcopy clinic](/preview/png/3920341.png)
ObjectivesTo describe the type-specific prevalence of anal and cervical human papillomavirus (HPV) infections and the cytology in HIV-negative women without a history of cervical cancer, attending a colposcopy clinic. To examine if an HPV positive anal smear is related to anal pathology and consequently indicative for further examinations (high resolution anoscopy, anal biopsy).Study designFrom 149 consecutive women an anal swab and a cervical swab were taken, using the Cervex-Brush®. The presence of 18 different HPV genotypes was determined using TaqMan-based real-time quantitative PCR targeting type-specific sequences of viral genes. From the fluid containing the cellular material, a liquid-based cytology sample was prepared of both collections with the robotic BD PrepStain™ Slide Processor. All slides were pre-screened by BD FocalPoint™ system and categorized from quintiles 1 to 5 and afterwards screened using targeted microscopic interpretation of selected suspicious fields using FocalPoint® guided screening review stations. The 2001 Bethesda System Terminology was used for the anal slides.ResultsNinety-six anal samples and all 149 cervical samples were adequate. Overall presence of HPV in the anus was 56.3% and in the cervix 53.7%. Overall, cytological abnormalities were found in 10.8% of anal smears and in 32.8% of cervical smears. HPV genotypes were identified in 47 samples on both sites: partial or complete concordance was found in 85.1%. HPV types 6, 16 and 18 were found in 27.9% and in 26.6% of the anal and cervical samples, respectively. The top three HPV types in the anus were 16, 51 and 39; in the cervix 16, 39, 51 and 56 (a shared 3rd place). HPV type 11 was not found.ConclusionsThe presence of HPV genotypes is clearly multifocal in this study population of women attending a colposcopy clinic, with high concordance of genotypes. The number of anal HPV infections is high. Although cytological abnormalities are rare, the presence of HPV may lead to anal lesions later in life. From this perspective, complementary medical history and clinical examination of the anal region are advised.
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology - Volume 164, Issue 1, September 2012, Pages 69–73