کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3920944 | 1599843 | 2011 | 5 صفحه PDF | دانلود رایگان |
ObjectivesA systemic inflammatory response after intrauterine funisitis is assumed to be an important priming factor for acute and chronic pulmonary morbidity and neurological impairment in premature infants. Fetal lymphocytes and monocytes modulate the primary immune response. Genetic regulation of the cytokine-mediated process is partially known. The objective of our study was to examine the pro-inflammatory and anti-inflammatory responses in umbilical cord blood mononuclear cells (CBMC) of preterm infants on the transcriptional level.Study designFifteen preterm infants with a gestational age ≤32 weeks were enrolled in this prospective study. Funisitis was diagnosed in five of the 15 by histological examination. Gene expression of pro-inflammatory cytokines (TNF-α, IL-8, IL-1β and IL-17) and anti-inflammatory cytokines (IL-10 and TGF-β1) was examined in CBMC by real time reverse transcription polymerase chain reaction.ResultsGene expression of IL-10 was significantly higher in the funisitis group compared to unexposed controls (p < 0.008). Expression of TGF-β1, TNF-α, IL-8 and IL-1β did not differ significantly between the funisitis and control group. IL-17 was detectable in only two samples.ConclusionsFunisitis is associated with increased IL-10 gene expression in CBMC of preterm infants with a gestational age ≤32 weeks. This might contribute to modulation of postnatal immunoreactions and immunoregulation in these individuals.
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology - Volume 155, Issue 1, March 2011, Pages 31–35