کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3921621 1599863 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Changes in expression of fibulin-5 and lysyl oxidase-like 1 associated with pelvic organ prolapse
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Changes in expression of fibulin-5 and lysyl oxidase-like 1 associated with pelvic organ prolapse
چکیده انگلیسی

ObjectivesPelvic organ prolapse is associated with defects in connective tissue including elastic fibers. The purpose of this study was to investigate expression of fibulin-5 and lysyl oxidase-like 1, which play an essential role in synthesis and assembly of elastic fibers in the uterosacral ligament, in samples taken from women with advanced pelvic organ prolapse compared with controls.Study designSpecimens were obtained prospectively during abdominal hysterectomy from 30 women with advanced pelvic organ prolapse and 30 controls matched to the study group for age and parity among postmenopausal women with benign gynecologic pathology. Expression levels of protein and mRNA of fibulin-5 and lysyl oxidase-like 1 in uterosacral ligaments were measured by Western blot analysis and real-time quantitative polymerase chain reactions. For statistical analyses, Student's t-test, chi-square test, Mann–Whitney U test, Fisher's exact test and Spearman's correlation were used.ResultsSignificant decrease in the expression of mRNA of fibulin-5 was found in patients with prolapse (P-value = 0.042; 0.743 ± 0.229 and 1.061 ± 0.537). The expression of mRNA of lysyl oxidase-like 1 was increased in the patient group (P-value = 0.017; 4.099 ± 2.832 and 1.816 ± 1.602). Similar results were shown in Western blot analysis. Differences in expression of fibulin-5 and lysyl oxidase-like 1 were noted according to stage of prolapse (P-value = 0.037 and 0.009).ConclusionsThere was decreased expression of fibulin-5 and increased expression of lysyl oxidase-like 1 in uterosacral ligaments in patients with pelvic organ prolapse, which suggests the possibility of defects in elastin synthesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology - Volume 145, Issue 1, July 2009, Pages 117–122
نویسندگان
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