کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3933543 1253353 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modulation of HOXA10 and other markers of endometrial receptivity by age and human chorionic gonadotropin in an endometrial explant model
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Modulation of HOXA10 and other markers of endometrial receptivity by age and human chorionic gonadotropin in an endometrial explant model
چکیده انگلیسی

ObjectiveTo characterize the endometrial response to human chorionic gonadotropin (hCG), as influenced by uterine age, using endometrial receptivity markers including HOXA10, vascular endothelial growth factor (VEGF), and glycodelin in an endometrial explant culture system.DesignIn vitro molecular biology research.SettingAcademic infertility clinic and molecular biology laboratory.Patient(s)Fourteen prospective recipients of egg donation (mean age, 44 ± 8 years).Intervention(s)Subjects received cyclical estrogen (E2) and progesterone (P4) and underwent an endometrial biopsy on day 7 of P4. Endometrial biopsy samples were cut into 1-mm3 pieces and cultured in Dulbecco's modified Eagle's medium/Ham's F-12 with E2 and P4, without (control) or with hCG (400, 4000, and 40,000 mIU/mL) on Millicell-CM inserts for 24 hours.Main Outcome Measure(s)Explant viability was assessed using immunohistochemistry (IHC). Semiquantitative polymerase chain reaction was performed to evaluate relative gene expression via mRNA levels of HOXA10, VEGF, and glycodelin.Result(s)Explant viability was confirmed on IHC by histology and Ki-67 staining, a marker of proliferation. HOXA10, VEGF, and glycodelin gene expression increased at all concentrations of hCG over those of controls. HOXA10 gene expression was inversely correlated with age (−0.08- ± 0.03-fold decrease in gene expression/year of age).Conclusion(s)The endometrial explant culture system is a promising model for the study of endometrial response as it maintains interactions among the stroma, glands, and epithelium. HOXA10, VEGF, and glycodelin all demonstrated increased gene expression in response to increasing hCG concentrations, supporting the role of hCG as a candidate protein for blastocyst-endometrial communication. Statistically significant associations between age and expression of HOXA10 provide novel evidence that uterine age may play a role in endometrial response on a molecular level.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 93, Issue 4, 1 March 2010, Pages 1255–1259
نویسندگان
, , ,