کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3934452 1253378 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cyclic QDE peptide increases fertilization rates and provides healthy pups in mouse
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Cyclic QDE peptide increases fertilization rates and provides healthy pups in mouse
چکیده انگلیسی

ObjectiveTo evaluate the effects of cyclic QDE peptide (cQDE), derived from sperm fertilin β (ADAM2), in mouse in vitro fertilization (IVF) and its harmlessness on pups after embryo transfer.DesignProspective in vivo and in vitro study in mice.SettingMurine model in an academic research environment in France.Animal(s)Normal B6CBF1 mice.Intervention(s)Indirect immunofluorescence was used to evaluate cQDE binding to oolemma. The IVF assays were run in presence of the species-specific tripeptide (cQDE at 100 μM), and embryos were transferred in pseudopregnant mice. Controls were obtained by natural mating and IVF in regular medium followed by embryo transfer.Main Outcome Measure(s)Evaluation of fertilization, litter size, pup fertility and health.Result(s)Biotinylated cQDE peptide binds to oocyte plasma membrane and increases gamete fusion in cumulus-intact IVF assay. Litter size as well as pup development after embryo transfer showed no statistically significant differences compared with controls. Pups born from embryos that were incubated with the peptide were as healthy and normally fertile as control mice over at least three generations.Conclusion(s)Cyclic QDE is harmless and improves mouse IVF pregnancy rates. A randomized prospective clinical trial to evaluate the beneficial effect of cyclic FEE on human IVF is required before its clinical use.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 91, Issue 5, Supplement, May 2009, Pages 2110–2115
نویسندگان
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