کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3935207 | 1253405 | 2012 | 8 صفحه PDF | دانلود رایگان |

ObjectiveTo investigate the developmental window in which E2 exposure produces irreversible changes in ovarian function resulting in polycystic ovary.DesignBasic experimental study.SettingUniversity animal laboratory.Animal(s)Thirty Sprague-Dawley rats were administered a single E2 valerate dose (10 mg/kg of weight) at 1, 7, 14, 21, or 30 days of age. Control rats were injected with the vehicle at 1 day of age. All rats were sacrificed at 6 months of age.Intervention(s)Observation of vaginal opening, estrous cyclicity by vaginal smears, and ovarian morphometry in the 6-month-old rat.Main Outcome Measure(s)Measurement of ovarian noradrenaline by high-performance liquid chromatography coupled with electrochemical detection, serum levels of LH by enzyme-linked immunoassay, P, androstenedione, and E2 by enzyme immunoassay.Result(s)Rats exposed to E2 at 1, 7, or 14 days of life did not show estrual cycling activity and maintained a polycystic ovary (PCO) condition throughout the entirety of the study. However, if the exposure to E2 occurred after postnatal day 21, the PCO-induced condition was reversible. In rats that developed a permanent PCO condition, we observed significant effects of E2 on ovarian morphology if exposure occurred on postnatal day 1 and a presumable effect on the hypothalamus if the exposure occurred between postnatal days 1 and 14.Conclusion(s)Our findings suggest that in rats, the most sensitive period for the promotion of an irreversible PCO morphology by estrogenic compounds is during neonatal early follicular development.
Journal: Fertility and Sterility - Volume 98, Issue 5, November 2012, Pages 1283–1290