کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3935316 1253410 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recording and sorting live human sperm undergoing acrosome reaction
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Recording and sorting live human sperm undergoing acrosome reaction
چکیده انگلیسی

ObjectiveTo develop and evaluate a method to detect acrosome reaction (AR) in live human sperm.DesignProspective study.SettingBasic research laboratory.Patient(s)Human semen samples with normal parameters obtained from healthy donors.Intervention(s)Acrosome reaction assays.Main Outome Measure(s)Fluorescence assessment of AR.Result(s)Evaluating acrosomal exocytosis in live human sperm is challenging. In this study, we report that in reacting sperm, Pisum sativum agglutinin conjugated to fluorescein isothiocyanate rapidly permeates into the acrosome when fusion pores open and stabilizes the acrosomal matrix, preventing the dispersal of the granule contents.Conclusion(s)Fluorescent Pisum sativum agglutinin can be used to visualize AR in real time, to determine the percentage of sperm undergoing exocytosis upon stimulation, and to separate the population of reacting sperm by flow cytometry.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 97, Issue 6, June 2012, Pages 1309–1315
نویسندگان
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