Injection of day 2 embryo culture supernatant into the uterine cavity did not improve the pregnancy rate of day 3 embryo transfer in patients who underwent in vitro fertilization-embryo transfer: a randomized clinical trial

ObjectiveTo evaluate the effectiveness of uterine cavity injection of day 2 embryo culture supernatant before day 3 embryo transfer in patients who are undergoing in vitro fertilization-embryo transfer.DesignRandomized controlled trial.SettingA reproductive medical centre.Patient(s)A total of 90 infertile women, 45 of them in the study group and 45 in the control group.Intervention(s)Uterine cavity infection of day 2 embryo culture supernatant before day 3 embryo transfer.Main Outcome Measure(s)Embryo implantation rate and pregnancy rate.Result(s)The pregnancy versus implantation rates in the study group and control group were 48.9% versus 44.4% and 27.3% versus 22.1%, respectively. Although both pregnancy and implantation rates in the study group were higher than those in the control group, no statistically significant differences were found in these two parameters.Conclusion(s)Injection of day 2 embryo culture supernatant into the uterine cavity cannot improve the implantation and pregnancy rates of day 3 embryo transfer.