کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3936821 | 1253461 | 2013 | 9 صفحه PDF | دانلود رایگان |

ObjectiveTo investigate whether ghrelin signaling is involved in the pathogenesis of male factor infertility induced by leptin deficiency.DesignExperimental study.SettingUniversity academic medical center.Animal(s)Ten-week-old C57BL/6J mice and ob/ob mice.Intervention(s)Western blotting, (quantitative) reverse transcription–polymerase chain reaction (qRT-PCR), immunohistochemistry, and in situ end labeling of fragmented DNA.Main Outcome Measure(s)Expression levels of ghrelin and its functional receptor growth hormone (GH) secretagogue receptor 1a (GHS-R1α) were examined by Western blotting and immunohistochemistry. Ob/ob mice were injected IP with specific GHS-R1α antagonist, and thereafter germ cell apoptosis and steroidogenic capability were assessed by TUNEL assay, (q) RT-PCR, and radioimmunoassay.Result(s)Expression of GHS-R1α and its endogenous ligand ghrelin was both up-regulated in ob/ob testis. Inhibition of the ghrelin pathway restored androgen synthesis, reduced germ cell apoptosis, and thereby resulted in improved sperm production in ob/ob mice.Conclusion(s)Ghrelin, as an antagonistic partner of leptin in the endocrinic/paracrine circuit, may be involved in the pathogenesis of male factor infertility induced by leptin deficiency.
Journal: Fertility and Sterility - Volume 99, Issue 3, 1 March 2013, Pages 918–926