Improvement of in vitro culture of mouse cumulus-oocyte complexes using PDE3-inhibitor followed by meiosis induction with epiregulin

Presence of PDE3-I in cultured COCs improves survival. Washout of PDE3-I is necessary for meiotic resumption to occur. Epiregulin by itself induces meiotic maturation but is a weak inducer of progesterone production.