کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3938818 1253542 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quadruplex real-time polymerase chain reaction assay for molecular diagnosis of Y-chromosomal microdeletions
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Quadruplex real-time polymerase chain reaction assay for molecular diagnosis of Y-chromosomal microdeletions
چکیده انگلیسی

ObjectiveTo develop a rapid and reliable method for molecular diagnosis of Y-chromosomal microdeletions.DesignStudy of diagnostic accuracy.SettingMolecular diagnostics laboratories in three hospitals.Patient(s)A total of 701 men with nonobstructive azoospermia or oligozoospermia from three hospitals.Intervention(s)We developed a quadruplex real-time polymerase chain reaction (PCR) assay and evaluated its performance in molecular diagnosis of Y-chromosomal microdeletions.Main Outcome Measure(s)Analytic sensitivity, analytic specificity, clinical sensitivity, and clinical specificity.Result(s)The limit of detection of quadruplex real-time PCR assay was 100 pg genomic DNA. The method attained 100% analytic specificity, 100% clinical sensitivity, and 100% clinical specificity.Conclusion(s)We have successfully upgraded the diagnostic method published by the European Academy of Andrology and the European Molecular Genetics Quality Network. Our method was validated to be fast, simple, contamination free, of high analytic sensitivity and specificity. Therefore, it is strongly suggested that such quadruplex real-time PCR assay can be readily applied as clinical routine in the near future.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 97, Issue 4, April 2012, Pages 864–869
نویسندگان
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