کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3938839 | 1253542 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A tissue-engineered human endometrial stroma that responds to cues for secretory differentiation, decidualization, and menstruation A tissue-engineered human endometrial stroma that responds to cues for secretory differentiation, decidualization, and menstruation](/preview/png/3938839.png)
ObjectiveTo show the responsiveness of tissue-engineered human endometrial stroma to combinations of hormones that mimic the secretory and menstrual phases of the cycle.DesignIn vitro experimental study.SettingUniversity uterine biology research laboratory.Patient(s)None.Intervention(s)Telomerase immortalized human endometrial stromal cells cultured in monolayers (two-dimensional, 2D) or encapsulated in a collagen I hydrogel (three-dimensional, 3D) to create a simplified tissue-engineered stroma were exposed to hormone treatments mimicking early and late secretory phases, decidualization, and steroid withdrawal conditions to recapitulate menstruation.Main Outcome Measure(s)Morphologic and biochemical markers of decidualization and collagenase activity.Result(s)The 3D tissue can manifest changes in morphology and biochemical markers of decidualization similar to 2D culture and characteristic of endometrial stroma in vivo. Unlike 2D culture, the 3D tissue responded to steroid withdrawal by increased collagenase activity and tissue breakdown.Conclusion(s)Three-dimensional tissue-engineered endometrial stroma can mimic secretory and menstrual phases of the cycle and may be useful for studying uterine receptivity and menstruation in a physiological endocrine environment.
Journal: Fertility and Sterility - Volume 97, Issue 4, April 2012, Pages 997–1003