کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3939086 1253548 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Relationship between time of reperfusion and E-selectin expression, neutrophil recruitment, and germ cell apoptosis after testicular ischemia in a rat model
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Relationship between time of reperfusion and E-selectin expression, neutrophil recruitment, and germ cell apoptosis after testicular ischemia in a rat model
چکیده انگلیسی

ObjectiveTo examine the relationship between the time of reperfusion and neutrophil recruitment, E-selectin expression, and germ cell apoptosis in the ischemic and contralateral testis after testicular ischemia-reperfusion (IR) injury in a rat.DesignLaboratory study.SettingResearch laboratory in a faculty of medicine at Technion–institute of technology in Israel.Animal(s)Sixty adult Sprague-Dawley rats weighing 250–280 g.Intervention(s)Testicular IR.Main Outcome Measure(s)Testicular germ cell apoptosis was assessed by terminal deoxynucleotide transferase–mediated 2′-deoxyuridine 5′-triphosphate nick-end labeling immunohistochemical assay, using an in situ cell death detection kit. The recruitment of polymorphonuclear (PMN) cells was calculated per 100 venules. Expression of E-selectin was determined by using immunohistochemical analysis.Result(s)E-selectin expression and polymorphonuclear-cell recruitment in the contralateral testis increased significantly after 1 hour of reperfusion and then remained unchanged during the first 24–48 hours, followed by a gradual decrease. Germ cell apoptosis in the contralateral testis increased after 6 hours of reperfusion, achieved statistical significance after 24 hours, and decreased after 72 hours of reperfusion.Conclusion(s)Germ cell apoptosis in the contralateral testis increases most significantly within the first 24–48 hours, followed by a gradual decrease, after IR injury. E-selectin expression and neutrophil recruitment increases within the first 6 hours and apparently may initiate the increase in germ cell apoptosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 90, Issue 4, Supplement, October 2008, Pages 1517–1522
نویسندگان
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