کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3940161 1253580 2007 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Distinct sets of developmentally regulated genes that are expressed by human oocytes and human embryonic stem cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Distinct sets of developmentally regulated genes that are expressed by human oocytes and human embryonic stem cells
چکیده انگلیسی

ObjectiveTo identify genes that are expressed differently during final oocyte maturation and early embryonic development in humans.DesignComparison of gene expression profiles of human germinal vesicle oocytes (hGVO), human embryonic stem cells (hESC) and human foreskin fibroblasts.SettingResearch centers and a fertility unit in a university hospital.Patient(s)Fifty-five healthy women donated 76 hGVO.Intervention(s)None.Main Outcome Measure(s)Gene expression profiles were analyzed and compared with the use of microarray and reverse-transcription polymerase chain reaction.Result(s)Altogether, 10,183 genes were expressed in hGVO, and 45% of these genes were unclassified by biologic function. Four oocyte-specific genes (MATER, ZAR1, NPM2 and FIGLA) were detected in hGVO for the first time. Known components of 4 signaling pathways (MOS-MPF, transforming growth factor-beta, WNT, and NOTCH) were also found expressed in hGVO, with some components detected in hGVO for the first time. Distinct sets of genes that were revealed by comparison of expression profiles between hGVO, hESC, and human foreskin fibroblasts appear to be involved in oocyte maturation and early embryonic development.Conclusion(s)We obtained, for the first time, a large amount of information on gene expression of hGVO as compared with hESC. These data, from a unique research material—human oocytes, can now be used to understand the molecular mechanisms of early human development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 87, Issue 3, March 2007, Pages 677–690
نویسندگان
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