کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3940721 | 1253593 | 2006 | 8 صفحه PDF | دانلود رایگان |
ObjectiveTo investigate effects of delta-9-tetrahydrocannabinol (THC) on human sperm function in vitro.DesignLaboratory analysis of sperm motility after exposure to THC using computer-assisted semen analysis and acrosome reaction by fluoroscein isothiocyanate–labeled peanut agglutinin staining.SettingAn assisted reproductive technology unit.Patient(s)Seventy-eight male patients.Intervention(s)Sperm were divided into 90% (the best fertilizing potential used in assisted conception) and 45% (the poorer subpopulation) fractions by density centrifugation and incubated with THC at concentrations equivalent to therapeutic (0.032 μM) and recreational (0.32 and 4.8 μM) plasma levels at 37°C for 3 h.Main Outcome Measure(s)Sperm motility and spontaneous and induced acrosome reactions.Result(s)Percentage progressive motility was decreased dose dependently in the 90% fraction (by 2%–21%; P<.05; P<.001). The 45% fraction showed a greater decrease in percentage progressive motility (by 28% at 0.032 μM; 56% at 4.8 μM; P=.004 and P=.01 res). Straight line velocity and the average path velocity also were reduced (by 10%, in the 90% LAYER) in both fractions. Spontaneous acrosome reactions were reduced in the 90% (17% at 0.032 μM, 35% at 4.8 μM P=.004 and P<.001 resp) and more markedly in the 45% fractions (17%–35%; P<.001). When the acrosome reaction was artificially induced (90% fraction) by A23187, THC (4.8 μM) resulted in a 57% inhibition (P<.001).Conclusion(s)The use of THC as a recreational drug may adversely affect male fertility.
Journal: Fertility and Sterility - Volume 85, Issue 3, March 2006, Pages 653–660