کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3940853 | 1253597 | 2010 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Submucosal uterine leiomyomas have a global effect on molecular determinants of endometrial receptivity Submucosal uterine leiomyomas have a global effect on molecular determinants of endometrial receptivity](/preview/png/3940853.png)
ObjectiveTo evaluate the effect of uterine leiomyomas on the endometrium using molecular markers of endometrial receptivity: HOXA10, HOXA11, LIF, and BTEB1.DesignCase–control study.SettingUniversity medical center.Patient(s)Thirty reproductive-aged women with submucosal, intramural, or no uterine myomas who underwent hysteroscopy or hysterectomy.Intervention(s)Proliferative phase endometrial sampling was performed at the time of surgery. In uteri with a submucosal myoma, directed endometrial biopsies were obtained over the myoma and over normal myometrium.Main Outcome Measure(s)Endometrial HOXA10 expression was evaluated as a primary endpoint using quantitative real-time reverse transcriptase–polymerase chain reaction (RT-PCR) and immunohistochemistry. HOXA11, BTEB1, and LIF were evaluated using real-time RT-PCR.Result(s)Endometrial HOXA10 and HOXA11 messenger RNA (mRNA) expression were significantly decreased in uteri with submucosal myomas compared with controls and with uteri with intramural myomas. A similar trend was seen in BTEB1 mRNA expression; however, no difference was found in LIF mRNA expression. Immunohistochemistry localized the decrease in endometrial HOXA10 protein expression to stroma. In the presence of a submucosal myoma, there were no regional differences in gene expression.Conclusion(s)The molecular mechanism by which submucosal myomas adversely affect reproduction includes a global decrease in endometrial HOX gene expression, not simply a focal change over the myoma. This may explain the reproductive dysfunction observed with submucosal myomas.
Journal: Fertility and Sterility - Volume 93, Issue 6, April 2010, Pages 2027–2034