کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3940986 1253601 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Altered expression pattern of heat shock transcription factor, Y chromosome (HSFY) may be related to altered differentiation of spermatogenic cells in testes with deteriorated spermatogenesis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Altered expression pattern of heat shock transcription factor, Y chromosome (HSFY) may be related to altered differentiation of spermatogenic cells in testes with deteriorated spermatogenesis
چکیده انگلیسی

ObjectiveTo evaluate the expression patterns of heat shock transcription factor, Y chromosome (HSFY), in the testes showing deteriorated spermatogenesis.DesignProspective study.SettingUniversity hospital, its branch hospital, and academic laboratory.Patient(s)Men undergoing testicular biopsy for the investigation of infertility and men undergoing orchiectomy for testicular cancer.Intervention(s)After pathologic evaluation, specimens were subdivided into three groups: normal spermatogenesis (n = 8), maturation arrest (n = 5), and Sertoli cell–only syndrome (n = 4). Immunostaining and Western blotting techniques determined the expression of HSFY.Main Outcome Measure(s)Expression of HSFY in testes.Result(s)Western blotting data revealed HSFY in the testicular tissues with normal spermatogenesis, maturation arrest, and Sertoli cell–only syndrome, but the amount of the protein in the maturation arrest and Sertoli cell–only syndrome samples was altered. The immunohistochemical data demonstrated that HSFY was expressed in spermatogenic cells and Sertoli cells in all specimens. However, the expression of HSFY was low or absent in spermatogenic cells of maturation arrest specimens, and the ratio of HSFY expressed in Sertoli cells was different in the specimens with maturation arrest and with Sertoli cell–only syndrome.Conclusion(s)Altered expression of the HSFY in the testis showing deteriorated spermatogenesis may be associated with alteration of spermatogenic cell differentiation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 86, Issue 3, September 2006, Pages 612–618
نویسندگان
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