کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3941311 1253607 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fragmentation of DNA in morphologically normal human spermatozoa
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Fragmentation of DNA in morphologically normal human spermatozoa
چکیده انگلیسی

ObjectiveTo evaluate DNA fragmentation in spermatozoa with normal morphological appearance.DesignProspective study.SettingAcademic tertiary center.Patient(s)Fertile, subfertile, and infertile men were studied.Intervention(s)Terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate–fluorescein nick-end labeling assay and morphology assessment by phase contrast in the swim-up fractions.Main Outcome Measure(s)Simultaneous assessment of the percentage of normally shaped sperm and DNA fragmentation.Result(s)No DNA fragmentation was found in spermatozoa with normal morphology in any of the samples from the fertile group. In only one sample from the subfertile group did we observed normally shaped sperm cells exhibiting DNA fragmentation. However, in all the samples from the infertile group, we observed normal spermatozoa with DNA fragmentation. Spermatozoa from this late group exhibited a high proportion of DNA damage.Conclusion(s)In infertile men with moderate and severe teratozoospermia, the spermatozoa with apparently normal morphology present in the motile fractions after swim-up may have DNA fragmentation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 91, Issue 4, April 2009, Pages 1077–1084
نویسندگان
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