Visualization of human sperm chromosomes by using mouse oocytes cryothawed after enucleation

ObjectiveTo evaluate whether previously enucleated mouse oocytes that are vitrified are usable for the analysis of human sperm chromosomes.DesignProspective, comparative laboratory study.SettingAnimal breeding and reproduction department in a rural Japan university.Patient(s)Sperm samples were obtained from a fertile donor.Intervention(s)Human sperm were injected into previously enucleated–vitrified mouse oocytes.Main Outcome Measure(s)The rates of appearance of the human sperm chromosomes that were injected into previously enucleated–vitrified mouse oocytes.Result(s)The rates of appearance of human sperm chromosomes were 71%–72% in enucleated–vitrified mouse oocytes.Conclusion(s)The use of enucleated–vitrified mouse oocytes allowed visualization of human sperm chromosomes.