کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3944622 1254219 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hypoxia promotes vasculogenic mimicry formation by inducing epithelial–mesenchymal transition in ovarian carcinoma
ترجمه فارسی عنوان
هیپوکسی ترویج تشکیل مظهر عروق کرونری با القای انتقال پروتئین مزانشیمال در کارسینوم تخمدان
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
چکیده انگلیسی


• Hypoxia is critical to cell plasticity and functionally important for the formation of vasculogenic mimicry.
• Cellular plasticity of ovarian cancer cells was primarily reflected in morphological changes and their pluripotential ability.
• Exploring the role of hypoxia in vasculogenic mimicry may identify new therapeutic approaches.

ObjectivesThe functions of hypoxia and subsequent hypoxia-inducible factor-1α (HIF-1α) activation in vasculogenic mimicry (VM) are currently unclear. This study aimed to investigate the effects of hypoxia on VM formation in ovarian cancer, and explore the possible mechanism involved.MethodsThe expression levels of HIF-1α, E-cadherin, vimentin, Twist1, Slug, and VE-cadherin proteins were analyzed by immunohistochemistry in 71 specimens of epithelial ovarian cancer. The results were correlated with VM and survival analysis. We used a well-established in vitro model of a three-dimensional culture to compare VM formation under hypoxia and normoxia in ovarian cancer cell lines SKOV3 and OVCAR3. To explore the potential mechanism, we examined the effects of hypoxia on the mRNA and protein expression levels of both E-cadherin and vimentin.ResultsHIF-1α expression was correlated with loss of E-cadherin expression and up-regulated vimentin expression in 11 of the 18 VM-positive patients. Ovarian cancer with evidence of VM was significantly more likely to have high Twist1, Slug, and VE-cadherin expression levels. VM was observed in vitro under hypoxia. The ovarian cancer cells presented morphological epithelial–mesenchymal transition (EMT)-like changes (more fibroblastoid morphology and loss of cellular cohesiveness) under hypoxic conditions. The mRNA and protein levels demonstrated the induction of EMT after hypoxia. Clinicopathological analysis revealed that both VM and HIF-1α expression levels presented shorter survival durations.ConclusionsHypoxia contributed to VM formation by inducing EMT. These results may offer new insights for consideration in ovarian cancer treatment strategies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gynecologic Oncology - Volume 133, Issue 3, June 2014, Pages 575–583
نویسندگان
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