Immunohistochemical and molecular study of severe cervical dysplasia associated with HPV-83

BackgroundBased on epidemiological data, infections with specific types of HPV can be classified as high-risk (HR), low-risk (LR) or intermediate-risk (IR) HPV depending on the risk of progression to cervical cancer.CaseA 70-year-old woman consulted for relapse of abnormal cytology with high-grade squamous intraepithelial lesions (HSIL) associated with HPV-83 infection. Histological examination demonstrated high-grade cervical intraepithelial neoplasia (CIN III) with strong and diffuse staining by the P16INK4a-specific antibody.ConclusionThis patient’s intermediate-risk HPV infection (HPV-83) rapidly progressed to severe cervical intraepithelial neoplasia (CIN III) with strong anti-P16INK4a immunolabelling. Analysis of the E6 peptide sequence revealed several mutations in one of the two putative zinc finger regions (AA residues 107–135) associated with p53 binding.