Pubertal exposure to bisphenol A affects the reproduction of male mice and sex ratio of offspring

ObjectiveTo study the effects of pubertal exposure to bisphenol A (BPA) on the reproduction of male mice in adulthood and subsequent generation mice.MethodsMale mice aged 21 d were exposed to BPA at a dose of 50 mg/kg per day for 7 d by intraperitoneal injection. Sperm count, sperm deformity rate and testis histology were evaluated 35 d after exposure. Male fertility index and newborns were further observed by mating with the normal female mice.ResultsThe epididymal sperm number was decreased by 20.6% in BPA exposure group compared with the control (P<0.01). Sperm deformity rate in BPA group was increased by 9.65% compared with the control (P<0.05). Testis seminiferous tubules were abnormal with sloughing of germ cells; BPA exposure had no significant effects on the fertility of male mice in adulthood; sex ratio of male to female offspring was increased.ConclusionPubertal exposure to BPA disrupted spermatogenesis in adult mice, and the proportion of male offspring was increased compared with the control.