A Preliminary Study of The Short Circuit Current (Isc) Responses of Endometria from Estrum and Bilateral Ovariectomy Female Rats

ObjectiveTo investigate the property of the endometrium ion transport in the normal estruation and ovariectomize rats.MethodsThe basic electrophysiological property of the endometrium was carried out by mean of the short circuit current (Isc) recording with the rats.ResultsThe baseline Isc and the transepithelial resistance (Rte) were different between the estruation and ovariectomized groups. Apical application of Na+ channel blocker, amiloride (10 μmol/L), or CFTR Cl− channel agonist, forskolin (10 μmol/L) did not significantly affect the Isc in the two groups. However, the Isc in the model group was more sensitive to the CFTR Cl− channel blocker (glibenclamide, 1 mmol/L, apical) decreased about 27.0% (P<0.05, n=6), no remarkably changes in the estruation rats. While apical application CACC Cl− channel blocker, DIDS (4,4′-diisothiocyanostilbene-2,2′-disulfonic, 100 μmol/L), the Isc was much more sensitive, percentage almost to 36.52% (P<0.01) in the model group than that in the control. Furthermore, basolateral application of bumetanide (100 μmol/L), a blocker of Na+−K+−2Cl− cotransport, didn't significantly reduce the Isc in the two groups.ConclusionThe baseline Isc and membrane resistance in the ovariectomize group is much higher than that in the normal estruation group, and the endometrial epithelium Cl− secretion may be mediated by predominantly calcium-dependent Cl− channels (CACC) and activating adenylate cyclase and apical cAMP-dependent Cl− channels (CFTR) in the model group with minor contributions in the control. Furthermore, the endometrial epithelium responses to different stimulants exists difference in the control and model group a likely mechanism for the ovary hormone.