Expression of the Calbindin-d28k Protein and Gene in Human Ovary

ObjectiveTo investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary.MethodsThe expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) methods.ResultsPositive expression of the CaBP-d28k protein and mRNA was observed in human ovary. The ovarian expression of the CaBP-d28k protein appeared in the cytoplasm of oocytes, granulose cells, theca cells, and two types of cells of corpus luteum. From primordial follicle, primary follicle to secondary follicle, increased CaBP-d28k levels were showed (P<0.05), and the CaBP-d28k expression in secondary follicle had the highest level.ConclusionHuman ovarian tissue locally expresses CaBP-d28k, and its expression level increases with follicular development.