Investigation of Function of Novel Sperm Binding Protein HBRP in Human

ObjectiveTo investigate the biology function of novel protein related to bovine seminal plasma protein in human testis.MethodsRecombination pcDNA3/HBRP was constructed and transfected to HEK293 cell and permanently expression cell line was established. The activity of protein kinase C (PKC) of the cell line was detected by autoradiography method.ResultsThe stable expression cell line of HBRP was obtained. The HBRP inhibited the activity of PKC significantly.ConclusionOne of the new functions of novel sperm binding protein in human is the inhibitor action on activity of PKC. It may be involved in the sperm capacitation and acrosome reaction.