Differentially Gene Expression Profile Related to Inflammation in Endometrial Cells Induce by Lipopolysaccharide

ObjectiveTo investigate differentially expressed genes related to inflammation in endometrial cells induced by Lipopolysaccharide(LPS).MethodsNormal endometrium in the proliferative phase of specimen from 3 cases for the experiment was collected. The LPS group were treated with 50 µg/ml LPS. Total RNA was extracted using Trizol reagent from cells. RNA quality was assessed by determining the OD260/280 ratio by agarose gel electrophoresis, the chip was scanned by laser scanner. The acquired was analyzed.ResultsA total of differentially expressed genes were found, these genes were relative to many aspects. Among them, the expression of genes involved in inflammation were up-regulated by LPS, such as overexpression of IL-1β, 8, etc.ConclusionThe results indicates that inflammation-related genes may be one of the mechanisms of abnormal uterine bleeding by LPS-induced.