کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3970069 | 1600910 | 2015 | 8 صفحه PDF | دانلود رایگان |
• Subject was azoospermic reciprocal translocation 46, XY, t(5;7;9;13) carrier.
• H&E stained testicular sections revealed no spermatids or sperm in the patient.
• TUNEL assay demonstrated apoptotic cells in testicular sections of translocation carrier.
• Increase in zygotene and decrease in the pachytene spermatocytes were observed in subject.
• Significant decrease in the recombination frequency of the translocation carrier
Spermatocyte spreading and immunostaining were applied to detect meiotic prophase I progression, homologous chromosome pairing, synapsis and recombination in an azoospermic reciprocal translocation 46, XY, t(5;7;9;13)(5q11;7p11;7p15;9q12;13p12) carrier. Histological examination of the haematoxylin and eosin stained testicular sections revealed reduced germ cells with no spermatids or sperm in the patient. TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay showed apoptotic cells in testicular sections of translocation carrier. Immnunofluorescence analysis indicated the presence of an octavalent in all the pachytene spermatocytes analysed in the patient. Meiotic progression was disturbed, as an increase in zygotene (P < 0.001) and decrease in the pachytene spermatocytes (P < 0.001) were observed in the t(5;7;9;13) carrier compared with controls. It was further observed that 93% of octavalents were found partially asynapsed between homologous chromosomes. A significant decrease in the recombination frequency was observed on 5p, 5q, 7q, 9p and 13q in the translocation carrier compared with the reported controls. A significant reduction in XY recombination frequency was also found in the participants. Our results indicated that complex chromosomal rearrangements can impair synaptic integrity of translocated chromosomes, which may reduce chromosomal recombination on translocated as well as non-translocated chromosomes, a phenomenon commonly known as interchromosomal effect.
Graphical AbstractFigure optionsDownload high-quality image (84 K)Download as PowerPoint slide
Journal: Reproductive BioMedicine Online - Volume 30, Issue 6, June 2015, Pages 651–658