کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3971141 | 1256750 | 2009 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Ultrastructural markers of quality in human mature oocytes vitrified using cryoleaf and cryoloop Ultrastructural markers of quality in human mature oocytes vitrified using cryoleaf and cryoloop](/preview/png/3971141.png)
This study describes and compares the possible effects of vitrification on the ultrastructural morphology of 20 human mature oocytes vitrified using two different supports, cryoleaf (n = 10) and cryoloop (n = 10). Fresh human mature oocytes (n = 15) were used as controls. Fresh and vitrified–warmed oocytes appeared rounded, with a homogeneous cytoplasm, an intact oolemma and a continuous zona pellucida. Sparse microvacuolization was only occasionally detected in fresh and vitrified–warmed oocytes, to the same extent. About 50% of the vitrified oocytes contained atypical, small and slender mitochondria–smooth endoplasmic reticulum aggregates, whereas a non-homogeneous microvillar pattern was observable in only 30% of the oocytes subjected to vitrification, regardless of the support utilized. Cortical granule content appeared generally reduced after vitrification, but cryoleaf-supported oocytes contained more cortical granules than cryoloop-supported oocytes (P < 0.05). Thus good overall preservation and virtual absence of cytoplasmic vacuolization seem to be the most relevant markers of quality in vitrified–warmed oocytes, using either support. In addition, cryoleaf-supported oocytes retained a higher number of cortical granules than cryoloop-supported oocytes. The variety of ultrastructural alterations recorded emphasizes the need for further studies aimed at assessing the actual tolerance of human oocytes to vitrification.
Journal: Reproductive BioMedicine Online - Volume 19, Supplement 3, 2009, Pages 17-27