کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3975793 1600989 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Low-dose LBH589 increases the sensitivity of cisplatin to cisplatin-resistant ovarian cancer cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Low-dose LBH589 increases the sensitivity of cisplatin to cisplatin-resistant ovarian cancer cells
چکیده انگلیسی

ObjectiveThere is a need to develop alternative therapeutic strategies to overcome cisplatin-associated resistance in patients with ovarian cancer. Histone deacetylation (HDAC) associated with inactivation of genes has been implicated in the epigenetic silencing of tumor suppressor genes affecting critical biological activities in cancer cells and may be an important factor in acquired cisplatin-associated resistance. In this report, we tested a combination of cisplatin and LBH589 (histone deacetylation inhibitor) in cisplatin-resistant ovarian cancer cells to explore the reversal effect of cisplatin resistance and changes of gene expression.Materials and MethodsTo detect the synergistic effects of antiproliferation between cisplatin and LBH589 in ovarian cancer cells, we performed a cell viability assay and a clonogenic assay. To investigate the differences of gene expression between cells treated by cisplatin alone and cotreated with cisplatin and LBH589, a microarray mRNA analysis was performed.ResultsIn the presence of low-dose LBH589, the inhibition concentration value of cisplatin for A2780-cp70 cells was much lower than with cisplatin treatment alone. Gene expression profiles identified that a total of 354 genes had been significantly upregulated and a total of 63 genes been downregulated with LBH589 cotreatment.ConclusionWe hypothesized that combination of cisplatin and LBH589 can override cisplatin-associated resistance in ovarian cancer cells. These results provide initial evidence for testing this combination in clinical use.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Taiwanese Journal of Obstetrics and Gynecology - Volume 50, Issue 2, June 2011, Pages 165–171
نویسندگان
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