Evaluation of Polymerase Chain Reaction-Based Ribosomal DNA Sequencing Technique for the Diagnosis of Mycotic Keratitis

The PCR and rDNA sequencing-based approaches may be useful alternatives to culture-based techniques for the rapid and sensitive diagnosis of fungal keratitis. We found that clinically significant pathogenic fungal strains can be detected and differentiated by this method in a short period. This method therefore is useful for the early treatment of fungal infection.