Prevention of cisplatin ototoxicity: Efficacy of micronized flavonoid fraction in a guinea pig model

ObjectiveTo evaluate the effectiveness of micronized flavonoid fraction in preventing cisplatin ototoxicity in a guinea pig model.MethodsThis study was conducted on 23 guinea pigs in the Animal Laboratory of Izmir Atatürk Training and Research Hospital. Animals were divided into three groups: Group 1 consisted of eight animals receiving cisplatin only; Group 2 contained eight animals receiving cisplatin + micronized flavonoid fraction; and Group 3 contained seven animals that received micronized flavonoid fraction only. Their cochlear reserve was evaluated by measuring the distortion product otoacoustic emission on days 0 and 7.ResultsIn Groups 1 and 2, the intragroup signal–noise ratios were statistically different at all frequencies tested (based on negative ranks, p < 0.05). In Group 3, the intragroup signal–noise ratios did not differ significantly at the frequencies tested (p > 0.05). Comparison of the three groups showed statistically significant differences among the groups (p < 0.05). The post hoc Bonferroni correction showed statistically significant differences among all three groups (p < 0.016). The median signal–noise ratio of the three groups tended to increase (z-value was positive for all the frequencies tested; p < 0.01).ConclusionMicronized flavonoid fraction (Daflon) is effective against cisplatin-induced ototoxicity in guinea pigs.