کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4113714 | 1606091 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Interleukin-8 induction via NF-κB, p38 mitogen-activated protein kinase and extracellular signal-regulated kinase1/2 pathways in human peripheral blood mononuclear cells by Alloiococcus otitidis Interleukin-8 induction via NF-κB, p38 mitogen-activated protein kinase and extracellular signal-regulated kinase1/2 pathways in human peripheral blood mononuclear cells by Alloiococcus otitidis](/preview/png/4113714.png)
SummaryAlloiococcus otitidis is a newly recognized species of gram-positive bacteria frequently associated with otitis media. Although immunostimulating activity of this organism has been investigated, little is known about the signaling pathways of chemokine/cytokine induction by this organism. We investigated the role of NF-κB and mitogen-activated protein (MAP) kinases in chemokine interleukin-8 (IL-8) production by human peripheral blood mononuclear cells (PBMCs) after stimulation with A. otitidis. The organism could induce in vitro IL-8 production in human PBMCs. IκBα, NF-κB, p38 MAP kinase, p44/42 MAP kinase (ERK1/2) became phosphorylated in PBMCs after stimulation with A. otitidis. And, inhibitors of NF-κB (caffeic acid phenylethyl ester), p38 (SB 203580), or ERK1/2 (PD 98059) significantly reduced IL-8 induction by the organism. These results were similar to findings in IL-8 induction by Streptococcus pneumoniae, another gram-positive major middle ear pathogen. Our preliminary study suggests that multiple pathways including NF-κB, p38, and ERK1/2 were simultaneously activated, and were associated with IL-8 induction by A. otitidis in human PBMCs.
Journal: International Journal of Pediatric Otorhinolaryngology - Volume 71, Issue 9, September 2007, Pages 1465–1470