کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4155596 1273751 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Skin-derived precursors generate enteric-type neurons in aganglionic jejunum
ترجمه فارسی عنوان
پیشگام های حاصل از پوست، نورون های ضد باکتری را در ژژونوم آگنگلیونی تولید می کنند
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پریناتولوژی (پزشکی مادر و جنین)، طب اطفال و بهداشت کودک
چکیده انگلیسی

PurposeSkin-derived precursor cells (SKPs) may regenerate the enteric nervous system in Hirschsprung’s disease. SKPs migrate and differentiate into myenteric ganglia in aganglionic intestine. We sought to characterize the time-course of SKP gangliogenesis and enteric neurotransmitter synthesis in vivo.MethodsAdult Lewis rat jejunal segments were isolated and denervated with benzalkonium chloride (BAC). Denervation was evaluated by immunohistochemical (IHC) stains for markers of mature neuronal and glial cells. Green fluorescent protein (GFP)-expressing neonatal rat SKPs were cultured in neuroglial-selective medium. SKPs were transplanted into aganglionic segments 65–85 days after BAC treatment. IHC was performed to identify glia, neurons, and neurotransmitter synthesis in GFP + cells between post-transplant days 1 and 28.ResultsAganglionosis was confirmed by IHC. On post-transplant days 1 and 2, GFP + cells were detected near injection sites within the muscularis propria. GFP + cell clusters were evident only between longitudinal and circular smooth muscle layers at post-transplant days 14, 21, and 28. These structures co-expressed markers of mature neurons and gliocytes. Several markers of neurotransmitter synthesis were detected in GFP + clusters at days 21 and 28.ConclusionSKPs are capable of enteric neuroglial differentiation in vivo. SKPs migrate to the intermuscular layer of aganglionic intestine within days of transplantation. Our observations suggest that SKPs are capable of generating enteric ganglia in aganglionic intestine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pediatric Surgery - Volume 49, Issue 12, December 2014, Pages 1809–1814
نویسندگان
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